Sulforaphane Loaded PEGylated Iron Oxide-Gold Core Shell Nanoparticles: A Promising Delivery System for Cancer Therapy

Amir Izadi, Hamidreza Kheiri Manjili, Leila Ma’mani, Elham Moslemi, Maedeh Mashhadikhan

Abstract


Herein we described a novel combination of sulforaphane (SF) with PEGylated gold coated Fe3O4 magnetic nanoparticles [(PEGylated Fe3O4@Au) NPs] to promote SF maintenance as an effective and promising anticancer drug. Physico-chemical properties of synthesized MNPs were assessed by X-ray diffraction, transmission electron microscopy, scanning electron microscopy, vibrating sample magnetometery, dynamic light scattering, and Fourier transform infrared spectroscopy. Our in-vitro study of the effects of SF loaded (PEGylated Fe3O4@Au) on cell viability in a human breast adenocarcinoma cell line (MCF-7) confirms that SF cytotoxicity augmented when it was loaded onto the (PEGylated Fe3O4@Au) NPs compared with to SF alone or bare NPs. Further results of our flow cytometry and real-time PCR analyses established that this combination increased therapeutic effects of SF to induce apoptosis and necrosis, in addition to inhibiting migration in MCF-7 cell line. Our future experiments may introduce Fe3O4@Au NPs as a smart delivery system for SF for use in preclinical and in-vivo studies.

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