ANTIBACTERIAL ACTIVITIES OF MILLIPEDES EXTRACTS AGAINST METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA)

George Antepim Pesewu, Maxwell Kelvin Billah, Christian Adofo, Douglas Kwang, Michael Adetokunbo Olu-Taiwo, and Samuel Osei-Djarbeng

Abstract


The use of animals and their products have over the years played enormous roles and served as efficient sources of new pharmaceuticals. The study evaluated the antibacterial activities of various solvent extracts of two species of millipede against methicillin-sensitive and -resistant Staphylococcus aureus (MSSA and MRSA). Millipedes collected were categorized into 3 groups (Group 1 = Ophistreptus guineensis S. alone, Group 2 = Pachybolus ligulatus V. alone, Group 3 = mixture of the two species), and their products extracted using chloroform, ethanol, and distilled water. Dry extracts of the millipedes were tested against the bacteria using agar-well and paper-disc diffusion assays. Minimum inhibitory and bactericidal concentrations (MIC and MBC) of the potent solvent extracts were also determined. The highest mean diameter of zones of inhibition (29 ± 0.07 mm) and (24 ± 0.15) mm against MSSA and MRSA were observed for the ethanol extracts of Group 3 (mixture of O. guineensis + P. ligulatus) by the agar well diffusion assay. MIC and MBC values ranging from 1.56-6.25 mg/ml and 6.25-25.0 mg/ml, for the ethanol extract of the Group 3 millipedes against MSSA and MRSA were found respectively. This study has shown that extracts of millipedes possess antibacterial substances that can be effective against MRSA in-vitro.

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References


[ 1 ] Anonymous, 2011. Animal Biodiversity. Zootaxa 3148, 159-164.

[ 2 ] Sierwald, P. and Bond, J.E., 2007. Current Status of the Myriapod Class Diplopoda (Millipedes): Taxonomic Diversity and Phylogeny. Annu Rev Entomol 52(1): 401-420.

[ 3 ] Kubo, T., Fujita, K., Nihei, K. and Masuoka, N., 2003. Non-antibiotic antibacterial activity of dodecyl gallate. Bioorg Med Chem 11, 573-580.

[ 4 ] Enright, M.C., 2003. The evolution of a resistant pathogen-the case of MRSA. Curr Opin Pharmacol 3, 474-479.

[ 5 ] Rod, L., Hoyt, K.S. and Hayes, K., 2007. Methicillin-resistant Staphylococcus aureus (MRSA) Infection. Adv Emerg Nurs J 29, 118-128.

[ 6 ] Hiramatsu, K., Katayama, Y. and Yuzawa, H., 2002. Molecular genetics of methicillin-resistant Staphylococcus aureus. Int J Med Microbiol 292, 67-74.

[ 7 ] Zhang, K., Sparling, J., Chow, B.L., Elsayed, S., Hussain, Z., Church, D.L., et al., 2004. New quadriplex PCR assay for detection of methicillin and mupirocin resistant and simultaneous discrimination of Staphylococcus aureus from coagulase-negative staphylococci. J Clin Microbiol 42, 4947-4955.

[ 8 ] Bambeke, F.V., Balzi, E. and Tulkens, P.M., 2000. Antibiotic efflux pumps. Biochem Pharmacol 60, 457-470.

[ 9 ] Gibbons, S., Oluwatuyi, M. and Kaatz, G.W., 2003. A novel inhibitor of multidrug efflux pumps in Staphylococcus aureus. J Antimicrob Chem 51, 13-17.

[ 10 ] Kumar, A. and Schweizer, H.P., 2005. Bacterial resistance to antibiotics: Active efflux and reduced uptake. Adv Drug Deliv Rev 57, 1486-1513.

[ 11 ] Cepeda, J.A., Whitehouse, T., Cooper, B., Hails, J., Jones, K. and Kwaku, F., 2005. Isolation of patients in single rooms or cohorts to reduce spread of MRSA in intensive-care units: Prospective two-centred study. Lancet 365, 295-304.

[ 12 ] Ghana News Agency (GNA, 2012). Management of the Korle Bu Teaching Hospital has closed, copied to the Ghana News Agency (GNA) in Accra on Saturday. Available at: www.ghananewsagency.org/.../K-Bu-children-s-emergency-ward-closed/?[Accessed on 28thJanuary 2012].

[ 13 ] Pesewu, G.A., Dogbe, R., Asmah, R.H., Olu-Taiwo, M.A. and Adjei, D.N., 2014. Prevalence and susceptibility profiles of methicillin-resistant Staphylococcus aureus (MRSA) in the University of Ghana Hospital, Legon, Accra, Ghana. Int J Pharm Bio Sci 5(3), (B)185-93.

[ 14 ] Wang, L. and Barret, J.F., 2007. Control and Prevention of MRSA infections. Methods Mol Biol 391, 209-225.

[ 15 ] Pemberton, R.W., 1999. Insects and other arthropods used as drugs in Korean traditional medicine. J Ethnopharmacol 65(3), 207-216.

[ 16 ] Clinical Laboratory Standard Institute (CLSI, 2007): Performance Standards for Antimicrobial Susceptibility Testing: Ninth Informational Supplement CLSI document M100-S9. CLSI, Wayne, PA.

[ 17 ] Andrews, J.M., 2005. BSAC Standard Disc Susceptibility Testing Method (version 4). J Antimicrob Chemother 56, 60-76.

[ 18 ] Cheesbrough, M., 2006. District Laboratory Practice in Tropical Countries Part 2. 2nded. New York: Cambridge University Press.

[ 19 ] Xylander, W.E.R., 2009. Antibacterial substances and characterization of the haemolymph of Chilopoda and Diplopoda (Myriapoda, Arthropoda). Soil Organisms 81(3), 413-429.

[ 20 ] Valderrama, X., Robinson, J.G., Attagalle, A.B. and Eisner, T., 2000. Seasonal anointing with millipedes in a wild primate: A chemical defense against insects? J Chem Ecol 26(12), 2781-2783.

[ 21 ] Zito, M., Evans, S. and Weldon, P.J., 2003. Owl monkeys (Aotus spp) self-anoint with plants and millipedes. Folia Primatol 74, 159-161.

[ 22 ] Weldon, P.G., 2004. Defensive anointing: Extending chemical phenotype and unorthodox ecology. Chemoecology 14, 1-4.

[ 23 ] Carroll, J.F., Kramer, M., Weldon, P.J. and Robbins, R.G., 2005. Anointing chemicals and ectoparasites: Effects of benzoquinones from millipedes on the lone star tick, Amblyomma americanum. J Chem Ecol 31(1), 63-64.

[ 24 ] Roncadori, R.W., Duffey, S.S. and Blum, M.S., 1985. Antifungal activity of defensive secretions of certain millipedes. Mycologia 72(2), 185-191.

[ 25 ] Ngbolua, K.N., Ngunde-te-Ngunde, S., Tshidibi, D.J., Lengbiye, M.E., Mpiana, T.P., Ekutsu, E.G., et al., 2014. Anti-sickling and antibacterial activities of extracts from a Congolese Diplopod (Tachypodoiulus sp., Arthropoda). J Adv Bot Zool 1(3), 1-5.


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