The Protective Effect Of Some Natural Antioxidants Against Azithromycin Induced Testicular Dysfunction In Rats

Abeer Eldakak, Abeer M. N. H.Ph.D


Objective: To investigate whether vitamin E, selenium, ß-carotene, anthocyanin and green tea extract have a possible protective effect against azithromycin (AZ)-induced testicular dysfunction. Design: Experimental study. Setting: Research Institute of Ophthalmology, Giza, Egypt. Patient(s): 3 -week-old weanling male albino rats (n = 54). Intervention(s): AZ was administered to rats at dose of 20mg/kg bw orally for 3 days every two weeks. Vitamin E, ß-carotene pigment, anthocyanin pigment, green tea extract, Se as sodium selenite and their mixture were administered with AZ by gavage daily for 4 months. Main Outcome Measure(s): Body and reproductive organ weights, spermatozoal activity, fructose contents, testosterone (T), interstitial cell-stimulating hormone (ICSH), lipid peroxidation and, antioxidant enzyme activities were investigated. Histopathological changes in reproductive organs examined. Result(s): Significant decreases in serum T and ICSH, reproductive organ weights, spermatozoal activity and fructose content were recorded due to AZ administration, whereas these parameters increased the antioxidants-treated groups. A significant decrease in Testicular MDA level and marked increases in GSH-Px, SOD, and CAT activities were observed in rats treated with different antioxidants. However, the most positive treatment was Se-treatment and the mixture of vitamin E with ß-carotene pigment. AZ-treatment led to adverse histopathologic changes in the testes, epididymis cuda, seminal vesicle and p rostate, whereas normal histopathological image of the studied reproductive parts were observed in groups treated with Se, Vitamin E and ß-carotene. Conclusion(s): Antioxidants have a protective effect against testicular toxicity caused by AZ. This protective effect of the antioxidants seems to be closely involved with the suppressing of oxidative stress.

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